The Board of Food Standards Australia New Zealand gives notice of the making of this variation under section 92 of the Food Standards Australia New Zealand Act 1991.  The variation commences on the date specified in clause 3 of this variation.

Dated 20 February 2020

Standards Management Officer

Delegate of the Board of Food Standards Australia New Zealand

1             Name

This instrument is the Food Standards (Application A1176 – Enzymatic Production of Steviol Glycosides) Variation.

2             Variation to standards in the Australia New Zealand Food Standards Code

The Schedule varies Standards in the Australia New Zealand Food Standards Code.

3             Commencement

The variation commences on the date of gazettal.

Schedule

[1]           Schedule 3 is varied by

[1.1]        omitting subsection S3—35(1), substituting

                   (1)      In this section:

prescribed rebaudiosides are:

                            (a)      rebaudioside D;

                            (b)      rebaudioside M; and

                            (c)      rebaudioside AM.

                            rebaudioside AM means the steviol glycoside with the chemical name: 13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester.

                   (1A)   This specification relates to a steviol glycosides preparation obtained from the leaves of the Stevia rebaudiana Bertoni plant.

[1.2]        omitting paragraph S3—35(2)(c), substituting

(c)        by enzymatic conversion of purified stevia leaf extract to produce rebaudioside D using a protein engineered enzyme that:

(i)             contains both UDP‑glucosyltransferase (EC 2.4.1.17) and sucrose synthase (EC 2.4.1.13) components; and

(ii)            is sourced from Pichia pastoris strain UGT-A;

(d)        by enzymatic conversion of purified stevia leaf extract to produce one or more prescribed rebaudiosides using a combination of enzymes that contains:

(i)             a UDP-glucosyltransferase from Stevia rebaudiana sourced from Escherichia coli; and

(ii)            a UDP-glucosyltransferase from Solanum lycopersicum sourced from Escherichia coli; and

(iii)           a sucrose synthase (EC 2.4.1.13) sourced from Escherichia coli.

[1.3]        omitting paragraph S3—35(4)(a), substituting

                            (a)                Description—white to light yellow powder, approximately 150 to 300 times sweeter than sucrose;

[2]           Schedule 18 is varied by

[2.1]        inserting in the table to subsection S18—9(3), in alphabetical order

[2.2]        inserting in the table to subsection S18—9(3), in alphabetical order

[2.3]        inserting in the table to subsection S18—9(3), in alphabetical order